Chlorogenic acid alters ileal microbiota and metabolites in broiler chickens under immune stress

TL;DR

Chlorogenic acid helps reduce immune stress in chickens by changing gut bacteria and metabolites, which could lead to better stress management strategies.

Contribution

This study reveals that chlorogenic acid alleviates immune stress in broilers by modulating ileal microbiota and specific metabolites.

Findings

Chlorogenic acid increased beneficial bacteria like Clostridiaceae and Candidatus Arthromitus in immune-stressed broilers.

CGA reduced harmful bacteria such as Lachnospiraceae and Desulfovibrionaceae and lowered inflammation-related metabolites.

CGA enhanced antioxidant capacity by increasing metabolites like pyroglutamic acid and biliverdin.

Abstract

Immune stress in broilers can cause severe economic losses, and chlorogenic acid (CGA) is an effective plant extract for alleviating immune stress. This study investigated the effects of CGA on the intestinal microbiota and metabolites of broilers under immune stress. A group of 312 broiler chicks was randomly divided into four treatment groups: Saline control, lipopolysaccharide (LPS), LPS + CGA (LCGA), and Saline + CGA (SCGA). The SCGA and LCGA groups were fed a basal diet supplemented with 1,000 mg/kg CGA throughout the whole experimental period. The LPS and LCGA groups were injected with 0.5 mg/kg LPS on days 14, 15, and 16 to induce immune stress. At day 17, the ileal contents were collected for analysis. Results showed that LPS-induced immune stress decreased the abundance of Subdoligranulum, thereby lowering microbial richness. However, dietary CGA increased beneficial bacteria like Clostridiaceae (P < 0.05) and Candidatus Arthromitus (P < 0.01) in immune-stressed broilers, while reducing harmful Lachnospiraceae (P < 0.05) and Desulfovibrionaceae (P < 0.05). Non-targeted metabolomics revealed that immune stress significantly increased methylmalonic acid, N-acetyl-L-aspartic acid, trans-ferulic acid, and pantothenic acid, while significantly downregulating pyroglutamic acid, L-glutamic gamma-semialdehyde, and biliverdin, indicating that immune stress (LPS) induced inflammation and oxidative damage. CGA addition reduced corticosterone, prostaglandin B2, trans-ferulic acid, and methylmalonic acid levels, while increasing pyroglutamic acid, L-glutamic gamma-semialdehyde, biliverdin, chenodeoxycholic acid, desmosterol, and 4-hydroxycinnamic acid, confirming that CGA can alleviate inflammation and enhance antioxidant capacity by influencing ileal microbiota and specific metabolite synthesis.

Our previous research indicated that CGA could effectively alleviate immune stress in broilers. However, it was unclear whether its antistress effects were achieved by altering the gut microbiota and metabolites in the ileum of immune-stressed broilers. In this study, CGA altered gut microbiota and metabolites associated with alleviating immune stress were provided, which will provide new insights into strategies to target gut microbiota and metabolites to relieve immune stress in broilers. These findings contribute to a better understanding of the mechanism of action of CGA on the attenuation of immune stress and provide new approaches to immune stress therapy by regulating gut microbiota and metabolites.

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