Proteomic Analysis of FFPE Tissue Samples Identifies Potential Molecular Mechanisms Mediating Resistance to Radiotherapy in Rectal Cancer
Tobias Zott, Michael Wolf, Günter Plessl-Walder, Heinz Regele, Michael Bergmann, Samuel M. Meier-Menches, Christopher Gerner, Gerd R. Silberhumer, Andrea Bileck
TL;DR
This study identifies proteins linked to resistance to radiotherapy in rectal cancer, which could help predict treatment response and guide personalized therapy.
Contribution
The study identifies novel proteins associated with radioresistance in rectal cancer using proteomic analysis of FFPE tissue.
Findings
1685 proteins were identified, with 221 differentially expressed in tumor vs. normal tissue.
Proteins like CEACAM, MCM complex components, and CD55 were upregulated in nonresponders to chemoradiation.
Autophagy and DNA repair-related proteins (e.g., TOM1, TP53BP1) distinguished non- and complete responders.
Abstract
Chemoradiation prior to surgery in locally advanced rectal cancer is the current standard therapy but is not effective in all rectal cancer patients. Prognostic markers supporting patient stratification with respect to clinical response would therefore be desirable. The aim of this study was to investigate pathophysiological mechanisms underlying radioresistance and to identify potential prognostic markers by comparative proteome profiling. Therefore, formalin fixed paraffin-embedded tissue (FFPE) samples from rectal tumors (n = 50) and normal control tissue (n = 39) of nonresponders and responders to neoadjuvant chemoradiation were analyzed. As a result, 1685 robustly identified proteins were further evaluated. Comparing tumor with corresponding control samples revealed 221 differentially expressed proteins (FDR < 0.05) with FTL, PCOLCE, and RCN3 being most striking in tumor tissue.…
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Taxonomy
TopicsCancer Research and Treatments · Cancer Immunotherapy and Biomarkers · Peptidase Inhibition and Analysis