Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel

TL;DR

This paper introduces a new 30-color flow cytometry panel to measure over 50 lymphocyte and monocyte populations in healthy adults, aiming to improve disease diagnosis.

Contribution

A novel 30-color spectral flow cytometry panel for comprehensive immunophenotyping of lymphocyte and monocyte subsets.

Findings

The panel measures over 50 distinct lymphocyte and monocyte populations from a single sample.

Data was collected from 148 healthy Australian adults to establish baseline immunophenotyping profiles.

Abstract

Lymphocytes play pivotal roles in disease pathogenesis and can be used as potential biomarkers for various immunological conditions. Yet, current flow cytometry methods used in clinical settings are often only capable of measuring between four to eight distinct lymphocyte populations. The purpose of our study was to measure many lymphocyte and monocyte populations from a single sample, with the long-term aim of validating our assay for diagnostic use in the Australian regulatory environment.

We designed and optimised a novel 30-colour lymphocyte immunophenotyping panel tailored for use on a 3-laser (V-B-R) spectral flow cytometer. This panel measures over 50 lymphocyte and monocyte populations.

In this report we present data derived from 148 healthy individuals.

This lays the groundwork for future clinical application of spectral flow cytometry tests and offers a more comprehensive approach to lymphocyte and monocyte analysis with future implications for disease diagnosis and monitoring.

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