# Hematological analysis of alpha-thalassemia: A single-center, retrospective clinical study

## TL;DR

This study identifies optimal blood test thresholds for detecting alpha-thalassemia in Fujian Province, China, to improve screening and genetic counseling.

## Contribution

The study provides region-specific cutoff values for MCV, MCH, and HbA2 for alpha-thalassemia screening in Fujian Province.

## Key findings

- The optimal cutoffs for MCV, MCH, and HbA2 were 77.85, 27.05, and 2.55, respectively.
- MCH had the highest diagnostic accuracy with an ROC area of 0.912 for alpha-thalassemia screening.
- The findings offer guidance for genetic counseling and prenatal diagnosis in the region.

## Abstract

To determine the optimal cutoffs of the three indicators (MCV, MCH and HbA2) for alpha-thalassemia screening and to evaluate the validity of these indicators in Fujian Province, China.

We conducted a retrospective analysis on the results of specimens received from May 2016 to April 2023. Receiver operating characteristic (ROC) curves were used to confirm the optimal cutoffs of the screening indicators. And the effectiveness of different combined screening methods was evaluated in patients with and without alpha-thalassemia.

The optimal cutoffs of MCV, MCH, and HbA2 were 77.85, 27.05 and 2.55, respectively. Among them, the area under the ROC curve of MCH was 0.912, and it was the best of the three parameters used for alpha-thalassemia screening.

The results can help clinicians and laboratory technicians perform genetic counseling and prenatal diagnosis for patients. It also provide a reference for alpha-thalassemia genotype distributions in our region and the optimal cutoff values of MCV, MCH and HbA2.