# Proteomic analysis of tumor cell nuclear expulsion reveals significant cell adhesion and RNA binding programs in extracellular chromatin

## TL;DR

This study explores proteins in expelled cancer cell nuclei and finds they promote tumor growth through adhesion and RNA binding proteins.

## Contribution

The study identifies unique proteomic signatures of TuNEPs and their role in tumor progression.

## Key findings

- TuNEPs contain enriched cellular adhesion molecules like integrin and basigin (BSG).
- Proteins in TuNEPs show increased citrullination linked to RNA binding.
- TuNEP proteins differ from those in neutrophil-derived NETs.

## Abstract

Understanding mechanisms of cancer cell death and the resulting effect on disease progression is crucial in cancer biology and the insight will likely offer better options for therapeutic treatment. Nuclear expulsion occurs in apoptotic cancer cells in a peptidylarginine deiminase 4 (Padi4) dependent manner. The resulting tumor cell nuclear expulsion product (TuNEP) promotes the outgrowth of neighboring cancer cells through chromatin-bound protein complexes. It is not clear what the protein compositions and functionalities are in these TuNEPs. In this study, we performed extensive proteomic profiling and identified key TuNEP protein components from mouse and human breast cancer cells as well as human lung cancer cells (4T1, MDA-MB-231, and PC9). We further compared TuNEP- specific proteins with those from apoptotic bodies or NETs from neutrophils. We found an enrichment of cellular adhesion molecules as well as increased citrullination of proteins associated with RNA binding. We showed that cellular adhesion molecules integrin and basigin (BSG) promote the growth of tumor spheroids. Our work revealed the unique TuNEP protein components distinct from neutrophil-derived NETs and shed light on potential mechanisms by which these cancer cell-derived TuNEPs promote tumor progression.

The online version contains supplementary material available at 10.1038/s41598-025-11807-z.