# Identifying new players of gynoecium development using tissue-specific transcriptome data of Arabidopsis **Authors:** Eliana Arias-Pérez, Valentín Luna-García, Judith J. Bernal-Gallardo, Stefan de Folter PMC · DOI: 10.1007/s00425-025-04784-0 · Planta · 2025-07-31 ## TL;DR This paper identifies new genes involved in reproductive development in Arabidopsis using tissue-specific transcriptome data. ## Contribution The study discovers novel genes affecting fertility and reproductive organ development in Arabidopsis. ## Key findings - Genes CLE19, TBL36, ATHB5, CYCP4;1, AT3G06035, and AT1G15760 affect fertility in Arabidopsis. - Mutant plants showed gynoecia with aborted ovules and short fruits with fewer seeds. - Pollen development and pollen tube growth were affected in most mutants. ## Abstract We identified new players of male and female reproductive development using tissue-specific gynoecium transcriptome data of Arabidopsis. Reproductive success in angiosperms depends on the correct development of the male and female organs. Pollen grains will land and germinate on the stigma, followed by the growth of pollen tubes that pass through the transmitting tract system of the gynoecium to reach the ovules. After the fertilization process occurred, seed and fruit development initiates. Genetic networks direct these biological processes needed for flower and fruit development to occur. Although many genes have been identified, still genes are to be discovered also to be involved in these networks. The availability of transcriptomic data from specific tissues of the gynoecium in Arabidopsis allowed us to select genes for functional analysis. As a result, from the analysis of the mutant plants we found that the genes CLE19, TBL36, ATHB5, CYCP4;1, AT3G06035, and AT1G15760 affect fertility in Arabidopsis. The mutant plants showed gynoecia with aborted ovules and short fruits with a lower number of seeds compared to wild type. Furthermore, pollen development and pollen tube growth were affected in most of the mutants. These results help us know and understand the genes that contribute to flower development in Arabidopsis. The online version contains supplementary material available at 10.1007/s00425-025-04784-0. ## Linked entities - **Genes:** CLE19 (CLAVATA3/ESR-RELATED 19) [NCBI Gene 822009], TBL36 (TRICHOME BIREFRINGENCE-LIKE 36) [NCBI Gene 824593], HB5 (homeobox protein 5) [NCBI Gene 836656], CYCP4;1 (cyclin p4;1) [NCBI Gene 819082], AT3G06035 (Glycoprotein membrane precursor GPI-anchored) [NCBI Gene 819776], AT1G15760 (Sterile alpha motif (SAM) domain-containing protein) [NCBI Gene 838145] - **Species:** Arabidopsis (taxon 3701) ## Full-text entities - **Genes:** BEE3 (BR enhanced expression 3) [NCBI Gene 843719] {aka BR enhanced expression 3, F25P22.25, F25P22_25}, CLE19 (CLAVATA3/ESR-RELATED 19) [NCBI Gene 822009] {aka ATCLE19, CLAVATA3/ESR-RELATED 19, EMBRYO SURROUNDING REGION 19, ESR19}, COBL11 (COBRA-like protein 11 precursor) [NCBI Gene 828819] {aka COBRA-like protein 11 precursor, T24A18.60, T24A18_60}, AT3G06035 (Glycoprotein membrane precursor GPI-anchored) [NCBI Gene 819776], BEE1 (BR enhanced expression 1) [NCBI Gene 838421] {aka BR enhanced expression 1, F15H18.11, F15H18_11}, LUG (transcriptional corepressor LEUNIG) [NCBI Gene 829390] {aka L23H3.30, L23H3_30, LEUNIG, RON2, ROTUNDA 2}, TAA1 (uncharacterized protein) [NCBI Gene 843393] {aka CKRC1, F24J13.13, F24J13_13, SAV3, SHADE AVOIDANCE 3, WEAK ETHYLENE INSENSITIVE 8}, CUC1 (NAC (No Apical Meristem) domain transcriptional regulator superfamily protein) [NCBI Gene 820748] {aka ANAC054, ATNAC1, Arabidopsis NAC domain containing protein 54, CUP-SHAPED COTYLEDON1}, LFY (floral meristem identity control protein LEAFY (LFY)) [NCBI Gene 836307] {aka LEAFY, LEAFY 3, LFY3, MAC9.13, MAC9_13}, SPT (basic helix-loop-helix (bHLH) DNA-binding superfamily protein) [NCBI Gene 829847] {aka AP22.25, AP22_25, SPATULA}, cycp3;1 (cyclin p3;1) [NCBI Gene 819115] {aka T14P1.11, cyclin p3;1}, AGP11 (arabinogalactan protein 11) [NCBI Gene 821093] {aka ARABINOGALACTAN PROTEIN 11, ATAGP11, F4P13.24, F4P13_24, arabinogalactan protein 11}, TRN2 (Tetraspanin family protein) [NCBI Gene 834713] {aka MZA15.11, MZA15_11, TET1, TETRASPANIN 1, TORNADO 2}, AGP6 (arabinogalactan protein 6) [NCBI Gene 831289] {aka F18O22.170, F18O22_170, arabinogalactan protein 6}, NTT (C2H2-type zinc finger family protein) [NCBI Gene 824936] {aka NO TRANSMITTING TRACT, WIP domain protein 2, WIP2}, TBL36 (TRICHOME BIREFRINGENCE-LIKE 36) [NCBI Gene 824593] {aka TRICHOME BIREFRINGENCE-LIKE 36}, COBL10 (COBRA-like protein 10 precursor) [NCBI Gene 821605] {aka COBRA-like protein 10 precursor}, ACT2 (actin 2) [NCBI Gene 821411] {aka DEFORMED ROOT HAIRS 1, DER1, ENHANCER OF LRX1 2, ENL2, FIZ2, FRIZZY AND KINKED SHOOTS 2}, HB5 (homeobox protein 5) [NCBI Gene 836656] {aka ATHB-5, ATHB5, MNA5.4, MNA5_4, homeobox protein 5}, CYCP4;1 (cyclin p4;1) [NCBI Gene 819082] {aka F16B22.23, cyclin p4;1}, AT1G20590 (cyclin) [NCBI Gene 838648] {aka F5M15.8, F5M15_8}, CLV3 (CLAVATA3) [NCBI Gene 817267] {aka AtCLV3, CLAVATA3, F12K2.17, F12K2_17}, CYCP3;2 (cyclin p3;2) [NCBI Gene 825226] {aka cyclin p3;2}, STK (K-box region and MADS-box transcription factor family protein) [NCBI Gene 826586] {aka AGAMOUS-like 11, AGL11, SEEDSTICK, T5L19.90, T5L19_90}, AT1G15760 (Sterile alpha motif (SAM) domain-containing protein) [NCBI Gene 838145] {aka F7H2.10, F7H2_10}, CES (basic helix-loop-helix (bHLH) DNA-binding superfamily protein) [NCBI Gene 839117] {aka CESTA, F4F7.28, F4F7_28, HAF, HALF FILLED}, PIN3 (Auxin efflux carrier family protein) [NCBI Gene 843432] {aka ARABIDOPSIS PIN-FORMED 3, ATPIN3, F15H11.14, F15H11_14, PIN-FORMED 3} - **Diseases:** CMM (MESH:D010437), male sterility (MESH:D007248), Septum (MESH:D000093665), PC (MESH:D056988), abortion (MESH:D000026), TPM (OMIM:602482), developmental defect (MESH:D000094602) - **Chemicals:** aniline blue (MESH:C017006), glycolipids (MESH:D006017), polysaccharides (MESH:D011134), water (MESH:D014867), acetic acid (MESH:D019342), fatty acids (MESH:D005227), chloroform (MESH:D002725), Technovit 7100 (MESH:C053012), alcohol (MESH:D000438), lipid (MESH:D008055), cytokinins (MESH:D003583), formaldehyde (MESH:D005557), Ca2+ (-), flavonoid (MESH:D005419), TRIzol (MESH:C411644), K2HPO4 (MESH:C013216), NaOH (MESH:D012972), ethanol (MESH:D000431), alcian blue (MESH:D000423) - **Species:** Moloney murine leukemia virus (no rank) [taxon 11801], Arabidopsis thaliana (mouse-ear cress, species) [taxon 3702] - **Cell lines:** S2 — Drosophila melanogaster (Fruit fly), Spontaneously immortalized cell line (CVCL_Z232), spt-12 — Mus musculus (Mouse), Hybridoma (CVCL_J992) ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC12313761/full.md ## Figures 5 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12313761/full.md ## References 1 references — full list in the complete paper: https://tomesphere.com/paper/PMC12313761/full.md --- Source: https://tomesphere.com/paper/PMC12313761