# Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates

## TL;DR

This study examines how the genetic diversity and virulence factors of Staphylococcus aureus in children have changed over 12 years, revealing insights into invasive infections and potential vaccine targets.

## Contribution

The study identifies newly invasive S. aureus clones and links specific virulence factors to invasive infections in pediatric populations.

## Key findings

- Invasive isolates showed reduced sequence type diversity compared to colonization isolates.
- CC8 and CC5 clonal complexes were associated with higher clinical severity scores.
- Staphylokinase and LukED were consistently linked to invasive infections over time.

## Abstract

Invasive Staphylococcus aureus infections cause high morbidity and mortality in children and adults. With rising antimicrobial resistance, optimal prevention strategies and novel therapeutics are needed. As an effective vaccine remains elusive, characterization of invasive isolates over time is required to identify determinants of invasive infection.

S. aureus isolates recovered from children with invasive infection and those with colonization were obtained. Isolates were examined by whole genome sequencing to evaluate gene repertoire, sequence type, clonal complex, and phylogenetic characterization, and isolate characteristics were correlated to clinical data.

118 children with invasive S. aureus infections were enrolled; 56% of infections were caused by methicillin-susceptible S. aureus (MSSA). Methicillin-resistance (MRSA) was associated with increased inflammation, though clinical outcomes of MRSA vs MSSA did not differ. Colonization isolates exhibited higher sequence type diversity than invasive isolates. Nine distinct clonal complexes (CC) were identified among all isolates; CC8 and CC5 were associated with higher clinical severity scores. Accessory gene regulator locus type 1, Panton-Valentine Leukocidin, and arginine catabolic mobile element declined over time. Staphylokinase and leukocidin ED were associated with invasive infection, while enterotoxin B was more frequent in colonizing isolates.

We observed a significant expansion in sequence type diversity among invasive clinical isolates over 12 years with the emergence of newly invasive clones in recent years. The presence of staphylokinase and LukED were associated with invasive infection over time. These findings provide insights into the pathogenesis of invasive S. aureus and may provide putative targets for immunologic approaches to prevention.