# Pilot study evaluating lipoma reduction with injected physiologic ice slurry

## TL;DR

A pilot study tested a coolant injection to reduce dog lipomas but found no significant shrinkage, though some cellular changes were observed.

## Contribution

The study introduces a novel coolant injection method for lipoma reduction and reports its feasibility and histopathologic effects.

## Key findings

- BXT-786 injection did not significantly reduce lipoma size over 12 weeks.
- Histopathologic changes suggested some impact on tumor cells.
- Adverse events were mostly unrelated to the coolant injection.

## Abstract

This study aimed to assess the adverse event profile following injection of canine lipomas with BXT-786 coolant, and to assess its impact on lipoma size.

Ten healthy adult client-owned animals, each with two similarly sized, cytologically confirmed lipomas were enrolled. Lipomas were injected with either BXT-786 coolant or control room temperature 0.9% saline solution. Two blinded, independent observers measured lipomas with a caliper and ultrasound. Dogs were reevaluated at 2, 4, 8, and 12-weeks post-injections to assess for adverse events and repeat measurements. Lipomas were surgically removed at 12 weeks post injection and submitted for histopathologic analysis. Health related quality of life was assessed using an owner completed questionnaire. Response to treatment was determined and adverse events were reported and graded.

Based on caliper assessment, majority of the lipomas were determined to be stable in size prior to surgical excision after BXT-786 or saline injection. Greater variability in response assessment was observed when using ultrasound. No statistically significant change was found in patient quality of life over an 8-week period. Adverse events were typically unrelated to BXT-786 injection, although one dog did develop cellulitis and mild necrosis in the lipoma.

Injection of BXT-786 coolant into lipomas was feasible and did induce some histopathologic changes consistent with effects on tumor cells, but it did not result in tumor shrinkage. Future studies could explore using coolants with more sustained coolant function and multiple injections to promote more efficient tumor reduction.