# The impact of thyroid hormone concentration fluctuations on colon cancer proliferation and growth

## TL;DR

This study shows that changes in thyroid hormone levels can influence the growth and spread of colon cancer, with lower hormone levels slowing cancer growth and increasing cell death.

## Contribution

The study demonstrates that reducing thyroid hormone levels can inhibit colon cancer proliferation and promote apoptosis in a mouse model.

## Key findings

- Hypothyroidism significantly reduced tumor growth and increased apoptosis in colon cancer cells.
- Hyperthyroidism did not significantly increase tumor growth compared to the control group.
- Ki67 expression was significantly lower in hypothyroid mice compared to control and hyperthyroid groups.

## Abstract

To examine the effects of thyroid hormone (TH) levels on colon cancer progression, HCT-116 colon cancer cells were inoculated into the axillary region of thymus-deficient male BALB/c nude mice. Mild hypothyroidism and hyperthyroidism were then induced to observe tumor growth patterns under different TH conditions.

Following subcutaneous tumor implantation, mice were randomly divided into three groups: hyperthyroid (levothyroxine-treated), hypothyroid (methimazole-treated), and control (saline-treated). Tumor volume and final mass were monitored throughout the study period. Excised tumors were subjected to histological analysis including hematoxylin-eosin (HE) staining, immunofluorescence, and TUNEL assay for apoptosis detection.

In the tumor-bearing experiment conducted with nude mice, the growth curve and tumor weight of the methimazole group exhibited a inhibitory trend compared to the saline group (P<0.05). In the immunofluorescence staining experiment, Ki67 expression was higher in control and hyperthyroid groups than hypothyroid (P<0.01 and P<0.001, respectively), with no significant control-hyperthyroid difference (P>0.05). TUNEL staining results demonstrated no significant presence of TUNEL-positive cells in the subcutaneous tumor tissue of the control group. Additionally, the proportion of TUNEL-positive cells in the subcutaneous tumor tissue of the levothyroxine group was slightly lower than in the control group. (P>0.05), whereas the proportion of TUNEL-positive cells in the methimazole group increased significantly (P<0.0001).

Fluctuations in thyroid hormone levels in the body can affect the proliferation and growth of colon cancer cells. Furthermore, reducing thyroid hormone levels can inhibit the proliferation of colon cancer cells while promoting their apoptosis.