# Protocol to quantify compound retention in Drosophila melanogaster for the pharmacokinetic study of orally administered compounds

## TL;DR

This paper introduces a new protocol called CORE to measure how fruit flies absorb and process orally administered compounds, improving pharmacokinetic studies.

## Contribution

The novel CORE protocol enables precise quantification of compound retention in Drosophila for pharmacokinetic research.

## Key findings

- CORE allows non-invasive quantitation of compound retention in fruit flies.
- The protocol supports simultaneous administration and analysis of multiple compounds.
- It addresses limitations in administering precise compound amounts in fruit flies.

## Abstract

Drosophila melanogaster is a valuable tool for intestinal function research. Here, we present a compound retention assay (CORE) to quantify the orally administered compounds that were absorbed, metabolized, or accumulated in the tissues (for example, fat) of D. melanogaster. We describe steps for preparing flies and media, compound administration, and sample collection. We then detail procedures for determining concentrations and calculating CORE values. This protocol circumvents the limitations of bioavailability research in the fruit fly, such as administering precise compound amounts.

For complete details on the use and execution of this protocol, please refer to Sadova et al.1

•Procedure for non-invasive compound retention quantitation in the fruit fly•Instructions for compound administration to the fruit flies for pharmacokinetic studies•Steps for the customization of compound solvents and extraction•Protocol that enables multiple compound administration and analysis simultaneously

Procedure for non-invasive compound retention quantitation in the fruit fly

Instructions for compound administration to the fruit flies for pharmacokinetic studies

Steps for the customization of compound solvents and extraction

Protocol that enables multiple compound administration and analysis simultaneously

Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Drosophila melanogaster is a valuable tool for intestinal function research. Here, we present a compound retention assay (CORE) to quantify the orally administered compounds that were absorbed, metabolized, or accumulated in the tissues (for example, fat) of D. melanogaster. We describe steps for preparing flies and media, compound administration, and sample collection. We then detail procedures for determining concentrations and calculating CORE values. This protocol circumvents the limitations of bioavailability research in the fruit fly, such as administering precise compound amounts.