Lambda Theta Reflectometry: A New Technique for Measuring Optical Film Thickness in Planar Protein Arrays
Alanna M. Klose, Joseph D. Katz, Robert Boni, David Nelson, Brian Hassard, Benjamin L. Miller
TL;DR
Lambda Theta Reflectometry (LTR) is a new method for measuring protein layers on surfaces with high precision, without using labels.
Contribution
LTR introduces a novel reflectometric technique that measures film thickness via null reflectivity at varying wavelengths and angles.
Findings
LTR achieves milli-Ångström precision in measuring SiO2 layer thickness.
LTR results align well with spectroscopic ellipsometry for film thicknesses between 1390 to 1465 Å.
LTR enables label-free biosensing across a wide range of protein concentrations without strict manufacturing requirements.
Abstract
Quantitative protein measurements provide valuable information about biological pathways, immune system functionality, and the mechanisms of disease. The most accurate methods for detecting proteins are label-free and preserve native protein-binding interactions. Label-free biomolecular interaction analysis includes reflectometry, a group of techniques that detect proteins by measuring the reflectance properties of a thin film on a substrate. Most of these techniques are limited in some way by instrument complexity, sensitivity, or consumable manufacturing requirements. To address these issues, we introduce Lambda Theta Reflectometry (LTR), a new reflectometric technique that measures changes in film thickness by determining the point of null reflectivity as a function of wavelength (lambda) and angle of incidence (theta). The substrate is simultaneously illuminated with a range of…
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Taxonomy
TopicsAdvanced Biosensing Techniques and Applications · Biosensors and Analytical Detection · Viral Infectious Diseases and Gene Expression in Insects