Correction to: Discovery of bifunctional diterpene cyclases/synthases in bacteria supports a bacterial origin for the plant terpene synthase gene family

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This is a correction to: Xinlu Chen, Meimei Xu, Jin Han, Mark Schmidt-Dannert, Reuben J. Peters, Feng Chen, Discovery of bifunctional diterpene cyclases/synthases in bacteria supports a bacterial origin for the plant terpene synthase gene family, Horticulture Research, Volume 11, Issue 10, October 2024, uhae221, https://doi.org/10.1093/hr/uhae221.

In the originally published version of this manuscript, our assignment of the product for the fused diterpene cylase–synthase (DCS) from Streptomyces sp. GS7 (StrDCS) was incorrect. Rather than syn-abieta-11,13(15)-diene, this is actually syn-abieta-7,13-diene, as first indicated by personal communication from Prof. Jeffrey Rudolf (Univ. Florida), based up on his investigations of a very closely related DCS (1), and supported by computational NMR shift prediction (2). Specifically, using the CHESHIRE CCAT webtool (http://cheshirenmr.info/index.htm), which revealed substantially greater divergence between predicted and observed chemical shifts for the previous double-bond isomer of syn-abietadiene [i.e., 11,13(15)] versus current [i.e., 7,13]. As a consequence, Figure 3 in this publication should be replaced by that shown here immediately below.

Figure 3. Bacterial DCS activity. Genes were recombinantly expressed in E. coli also engineered to produce GGPP, and enzymatic products extracted from the resulting induced cultures for analysis by GC-MS. Shown here are chromatograms and schemes indicating the relevant reactions for A) CseDCS, B) ChjDCS, and C) StrDCS.