Characteristic metabolite profile of 10 colorectal cancer-related bacteria

TL;DR

This study identifies unique metabolite profiles in 10 CRC-related bacteria, which may help explain how these microbes influence cancer progression.

Contribution

The study reveals distinct metabolite profiles and metabolic pathways in CRC-related bacteria, offering new insights into their role in cancer.

Findings

Lactobacillus strains show increased saccharides and organic acids like galactinol and glutaric acid.

Bifidobacterium strains exhibit significant changes in amino acids and derivatives such as glutamate and N-acetyl-5-hydroxytryptamine.

CRC-related pathogens like ETEC and Fn show elevated bile acids and polyamines, indicating altered metabolic pathways.

Abstract

Tumor metabolomics of colorectal cancer (CRC) is significantly different from normal tissues, due to nutrient deprivation, metabolite accumulation, acidity and hypoxia. Besides, gut microbiota has been confirmed to affect the progression of CRC. Microbiota metabolites might participate in the metabolic reprogramming of CRC cells and further regulate tumor microenvironment.

10 CRC-related strains are cultured in vitro (10 replicates per bacterium), including Enterotoxic Escherichia coli (ETEC), Peptostreptococcus anaerobius (Pa), Fusobacterium necroporum (Fne), Fusobacterium nucleatum (Fn), Lactobacillus plantarum (Lp), Lactobacillus acidophilus (La), Lactobacillus casei (Lc), Lactobacillus rhamnosus gg (LGG), Bifidobacterium bifidum (Bbi), Bifidobacterium Breve (Bbr). Bacterial culture supernatant is subjected to gas chromatography-mass spectrometry.

The 10 CRC-related strains have characteristic metabolite profiles, mainly referring to specific saccharides, amino acids, bile acids, polyamines and bioactive compounds. Saccharides and organic acids increase significantly in Lactobacillus (Lp, LA, Lc and LGG) compared with culture medium and other strains, such as galactinol, 1-ketose, beta-gentiobiose, glutaric acid, 3-phenyllactic acid, indlol-3-lactate. Chlorogenic acid, a beneficial polyphenol, increases significantly in Bbr. The abundance of amino acids and their derivatives changes significantly in Bifidobacterium (Bbi and Bbr), such as 2-hydroxy-2-methylbutanoic acid, N-acetyl-5-hydroxytryptamine and glutamate. Bile acids (lithocholic acid and cholic acid), polyamine (spermine), amino acids and derivatives (N-acetylaspartate, glutamate) increased significantly in the CRC-related pathogens (ETEC, Pa, Fn and Fne). Correspondingly, metabolic pathways are significantly affected, mainly including amino acid metabolism and nucleotide metabolism.

The 10 CRC-related strains possess significantly different metabolites and metabolic pathways. Specific metabolites and corresponding metabolic pathways might explain microbial CRC-promoting or -suppressing mechanisms.

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