Functional Identification and Transcriptional Activity Analysis of Dryopteris fragrans HMGR Gene
Meng Sun, Qian Ma, Xueqi Wang, Jialiang Guo, Jiaxuan Wang, Dongrui Zhang, Kirill Tkachenko, Wenzhong Wang, Ying Chang
TL;DR
This study identifies and analyzes HMGR genes in Dryopteris fragrans, showing their role in stress tolerance when overexpressed in tobacco plants.
Contribution
The study functionally characterizes D. fragrans HMGR genes and demonstrates their role in enhancing plant stress resistance.
Findings
Three HMGR genes (DfHMGR1/2/3) were identified and shown to be upregulated under salt and drought stress.
Transgenic tobacco plants overexpressing DfHMGRs exhibited enhanced drought and heat stress tolerance.
Promoter and co-expression analyses revealed regulatory regions and 21 potential transcriptional regulators.
Abstract
Dryopteris fragrans (L.) Schott synthesizes volatile sesquiterpenes through the mevalonate pathway (MVA), in which 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) serves as the key rate-limiting enzyme. Although HMGR plays a crucial role in terpenoid biosynthesis, its functional characteristics in D. fragrans and its involvement in stress responses remain unclear. This study identified three HMGR genes (DfHMGR1/2/3) from the transcriptome data of D. fragrans. Bioinformatics analysis revealed that the encoded proteins are localized to the endoplasmic reticulum and share high sequence similarity with fern homologs. Under abiotic stress conditions, DfHMGRs exhibited differential expression patterns, with marked upregulation under salt and drought stress. To validate the functions of these genes, we generated transgenic Nicotiana tabacum L. plants overexpressing DfHMGRs. Compared with…
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Taxonomy
TopicsPlant biochemistry and biosynthesis · Neurobiology and Insect Physiology Research · Plant Gene Expression Analysis