Revisiting the Genomic Epidemiology of Distinct Phage-Type Vibrio cholerae Strains Reveals Restricted Spatiotemporal Dissemination During an Epidemic

TL;DR

This study uses genome sequencing to track the spread and evolution of a specific cholera strain during an epidemic in China, revealing how it adapted to resist a phage.

Contribution

The study provides new insights into the genomic epidemiology and adaptive mutations of phage-resistant Vibrio cholerae strains during an epidemic.

Findings

PT6 strains formed an independent clone during the four-year epidemic and emerged in wave 2.

Most PT6 strains carried multiple CTXclassΦ genome copies and two copies each of RS1ET and RS1-4**.

Spontaneous mutations in the ompW gene were observed, enabling resistance to the VP5 phage.

Abstract

The El Tor biotype of Vibrio cholerae caused the seventh cholera pandemic (7CP). Although V. cholerae variants of this biotype frequently emerge, studies on their microevolution and spatiotemporal transmission in epidemics caused by a single clone are limited. During the cholera outbreak in Sichuan Province, China, in the 1990s, strains belonging to phage type 6 (PT6) but resistant to typing phage VP5 due to a deletion mutation in ompW, which is the gene associated with the VP5 receptor were identified. In this study, we analyzed PT6 strains using genome sequencing to reveal the genomic and transmission characteristics of such a transient phage type in China’s cholera epidemic history. The findings revealed that the PT6 strains formed an independent clone during the four-year epidemic and emerged in wave 2. Most of them carried multiple CTXclassΦ genome copies on chromosome 2 (Chr. 2) and two copies each of RS1ET and RS1-4** on chromosome 1 (Chr. 1). Frequent cross-regional transmission and local outbreaks within Sichuan Province, China, were revealed for this clone. A variety of spontaneous mutations in the ompW gene, conferring resistance to the VP5 phage, were observed under VP5 infection pressure, showing the incident mutation of OmpW for the survival adaptation of V. cholerae to phage pressure. Therefore, this genomic epidemiological revisit of these distinct phage-resistant phenotype strains reveals their clonal genetic structure, improves our understanding of the spread of V. cholerae by tracking their variation, and assists in epidemic source tracing and disease control.

Figures