# Transcriptome Analysis of Dimethyl Fumarate Inhibiting the Growth of Aspergillus carbonarius

## TL;DR

This study shows how dimethyl fumarate stops the growth of a harmful fungus that produces a dangerous toxin in fruits.

## Contribution

The study reveals the antifungal mechanism of dimethyl fumarate through transcriptome analysis of Aspergillus carbonarius.

## Key findings

- Dimethyl fumarate disrupts cell integrity and mycelium growth in A. carbonarius.
- 1402 differentially expressed genes were identified, including 987 up-regulated and 415 down-regulated genes.
- The compound obstructs secondary metabolism, reducing ochratoxin A production.

## Abstract

Aspergillus carbonarius is one of the main pathogens responsible for postharvest diseases in fruits and is also one of the main ochratoxin A-producing strains. It not only causes significant economic losses but also poses a risk to human health. We found an inhibitory effect of dimethyl fumarate fumigation on the growth of A. carbonarius. To further explore its antifungal mechanism, this study elucidated the functions of key pathway-related genes through a transcriptomics analysis. A total of 1402 differentially expressed genes (DEGs) were identified, including 987 up-regulated and 415 down-regulated genes. Dimethyl fumarate was found to significantly inhibit the growth of A. carbonarius by disrupting cell integrity and obstructing mycelium growth and secondary metabolism. These findings provide a basis for the potential application of dimethyl fumarate in the food industry to inhibit A. carbonarius and subsequent ochratoxin A contamination.