# Enhancing the Yield of Pleurotus ostreatus Through the Addition of Nucleotides and Nucleosides

## TL;DR

Adding nucleotides and nucleosides to the culture medium of Pleurotus ostreatus mushrooms boosts their yield, nutritional value, and enzyme activity.

## Contribution

This study introduces nucleotides and nucleosides as effective supplements for improving Pleurotus ostreatus cultivation.

## Key findings

- Nucleotides and nucleosides increased the yield and biological efficiency of Pleurotus ostreatus.
- Supplementation enhanced dietary fiber and amino acid content in the mushrooms.
- These substances significantly influenced the lignocellulolytic enzyme system.

## Abstract

Pleurotus ostreatus is a mushroom species renowned for its abundant nutritional and medicinal properties. Nevertheless, the yield of its fruiting bodies has long remained at a standstill, making it arduous to achieve substantial improvements. Because the traditional composting approach for enhancing the yield of Pleurotus ostreatus has drawbacks such as a long duration and a high susceptibility to mold contamination, incorporating nutritional supplements into the culture medium of P. ostreatus has emerged as a relatively straightforward yet effective approach to enhancing its yield. This study was predicated on the roles of nucleotides and nucleosides in cellular metabolism and signal transduction. These substances were applied during the cultivation process of P. ostreatus to investigate their impact on the growth and nutritional composition of this mushroom. The findings of this study demonstrate that the supplementation of nucleotides and nucleosides not only improved the yield and biological efficiency of P. ostreatus but also increased its dietary fiber content and amino acids. Furthermore, this research has disclosed that nucleotides and nucleosides exert a notable influence on the lignocellulolytic enzyme system. This investigation provides a scientific foundation for the development of novel yields—enhancing agents for P. ostreatus and offering new insights into cultivation techniques for the progress of P. ostreatus cultivation techniques in both academic and practical arenas.