# Effect of Dietary Supplementation of Biochars on Growth Performance, Bone Mineralization, Footpad Health, Lymphoid Organs Weight, Antibody Titers of Newcastle Disease and Infectious Bronchitis Disease in Broiler Chicks

**Authors:** Raheel Pervaiz, Riaz Mustafa, Umar Farooq, Waseem Abbas, Muhammad Farooq Khalid, Abdur Rehman, Munawar Hussain, Muhammad Muzammil Riaz, Asfa Fatima, Muhammad Aziz ur Rahman

PMC · DOI: 10.3390/vetsci12070680 · Veterinary Sciences · 2025-07-18

## TL;DR

Adding 1% biochar to broiler feed improves growth, bone health, footpad condition, and immunity against diseases.

## Contribution

This study demonstrates that biochar supplementation in broiler diets enhances growth and health outcomes in a controlled experimental setting.

## Key findings

- Dietary biochar improved body weight gain by 4.7% and reduced feed conversion ratio by 6.7%.
- Biochar enhanced tibia calcium and phosphorus content and improved footpad health and litter quality.
- Supplementation increased antibody titers against Newcastle disease and infectious bronchitis.

## Abstract

Use of agriculture by-products in animals feed as a feed additive not only ensures sustainable livestock production but also reduces the negative environmental impact of agriculture by-products. Our study provides evidence that dietary supplementation of 1% biochars improved body weight gain by up to 4.7%, reduced feed conversion ratio by 6.7% and enhanced tibia calcium and phosphorus content. Additionally, it improved footpad health, litter quality and titer against Newcastle disease and infectious bronchitis disease in broilers.

This study evaluated the effect of biochars on growth performance, nutrient digestibility, carcass yield, bone mineralization, litter quality and footpad lesions in broilers. Eight hundred day-old chicks were randomly divided into four treatments, 10 replicates per treatment (20 birds/replicate) for 35 days. Treatments were basal diet (control), a control diet with corncob (CC) biochar (1%), a control diet with wheat straw (WS) biochar (1%) and a control diet with sugarcane bagasse (SCB) biochar (1%). Body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) were recorded weekly. Nutrient digestibility, bone mineralization and carcass parameters were determined on the 21st and 35th days, while footpad lesions and litter quality were also assessed. The results revealed significant improvement (p < 0.05) in FI, BWG and FCR with supplementation. Nutrient digestibility was higher (p < 0.05) in the SCB biochar group. Tibia calcium and phosphorus levels were enhanced (p < 0.05) in the WS and SCB biochar groups, respectively. Footpad lesions were significantly lower (p < 0.05) in the CC biochar group, while litter quality was improved (p < 0.05) in the WS biochar group. Lymphoid organ relative weight results revealed that spleen weight was not affected by biochar supplementation in diet (p > 0.05), while dietary supplementation of CS and WS biochar in the diet resulted in the highest relative weights of thymus and bursa (p < 0.05). However, dietary supplementation of WS, SC and SCB biochar supplementation had affected positively the log value of the ND virus and IBV titers in birds. Overall, dietary supplementation of 1% biochars enhances growth performance, bone mineralization, footpad health immunity and litter quality in broilers.

## Linked entities

- **Diseases:** Newcastle disease (MONDO:0005875)

## Full-text entities

- **Diseases:** Infectious Bronchitis Disease (MESH:D003141), Footpad lesions (MESH:D009059), Newcastle Disease (MESH:D009521)
- **Chemicals:** calcium (MESH:D002118), phosphorus (MESH:D010758), Biochars (MESH:C540010), CC (-), CS (MESH:D002586)

## Full text

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## References

67 references — full list in the complete paper: https://tomesphere.com/paper/PMC12298835/full.md

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Source: https://tomesphere.com/paper/PMC12298835