# Automated Antithrombin Activity Detection with Whole Capillary Blood Based on Digital Microfluidic Platform

## TL;DR

This paper introduces a new automated device for quickly testing antithrombin activity using just a small blood sample, making it ideal for point-of-care use.

## Contribution

A novel digital microfluidic platform for automated antithrombin activity detection using whole capillary blood.

## Key findings

- The device requires minimal sample volume and eliminates plasma separation, saving time.
- Results are consistent with traditional methods, showing high reliability.
- The system is user-friendly and suitable for various clinical settings.

## Abstract

Antithrombin (AT) plays a crucial role in the human anticoagulant system and has extensive clinical applications. However, traditional detection methods often require large sample volumes, complex procedures, and lengthy processing times. Methods: We integrated digital microfluidics technology with AT detection to develop a point-of-care testing (POCT) device that is user-friendly and fully automated for real-time AT testing. Results: This device allows for automation and enhanced adaptability to various settings, requiring only a minimal sample volume (whole capillary blood), thereby omitting steps such as plasma separation to save time and improve clinical testing efficiency. Comparisons with conventional AT activity detection methods demonstrate a high degree of consistency in the results obtained with this device. Conclusion: The AT detection system we developed exhibits significant effectiveness and holds substantial research potential, positioning it to evolve into a clinically impactful POCT solution for AT assessment.