Selenium Metabolizing Capabilities of 12 Bacterial Strains Isolated from Urban Environmental Samples

TL;DR

Researchers found that Citrobacter bacteria efficiently remove selenium from water, suggesting they could be used as bioindicators in environmental studies.

Contribution

The study identifies Citrobacter spp. as dominant and efficient selenium-reducing bacteria in urban environments.

Findings

Citrobacter isolates removed over 95% of selenium from aqueous solutions within a week.

Citrobacter spp. directly reduced selenate to elemental selenium with minimal selenite intermediates.

Scandinavium hiltneri and Klebsiella aerogenes showed significantly lower selenium removal efficiency.

Abstract

The role of bacterial selenium metabolism in non-polluted environments remains underexplored within the selenium biogeochemical cycle. In this study, selenium-metabolizing bacteria were isolated from urban environmental samples. Among 12 isolates, 10 were identified as Citrobacter spp., while the remaining 2 were Scandinavium hiltneri and Klebsiella aerogenes. The Citrobacter isolates demonstrated high selenium-removal efficiency, removing over 95% of 5 mM selenium from the aqueous phase within one week. In contrast, S. hiltneri K24-1 and K. aerogenes K24-4 removed only 19% and 69%, respectively. A detailed investigation of five representative isolates, C. freundii K21-1, S. hiltneri K24-1, C. braakii K24-2, K. aerogenes K24-4, and C. freundii K24-5, revealed that Citrobacter spp. efficiently reduced selenate directly to elemental selenium, with minimal accumulation of selenite intermediates. These results highlight Citrobacter spp. as key selenium reducers and suggest their potential as bioindicators of selenium metabolic capacity in the environment.

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