Identification of a Novel Regulatory Gene, trmE, that Orchestrates Salmonella Flagellar Synthesis and Virulence
Haoyu Geng, Linyan Luo, Jian Zhang, Jingying Gao, Shizhong Geng, Paul Barrow
TL;DR
This study identifies a new gene, trmE, in Salmonella that controls flagellar production and affects the bacteria's ability to cause disease.
Contribution
The novel regulatory gene trmE is shown to influence flagellar synthesis and virulence in Salmonella Enteritidis.
Findings
trmE gene deletion reduces motility and flagellar gene expression in Salmonella.
trmE mutant shows decreased adhesion, invasion, and inflammatory response in macrophages.
trmE deletion leads to reduced mortality and inflammation in infected mice.
Abstract
It is well established that flagella play a critical role in bacterial motility and virulence, and the genes associated with flagellar synthesis and regulation have been extensively characterized. In this study, we identified the trmE gene as a novel modulator of flagellar synthesis in Salmonella Enteritidis. A transposon (Tn5) mutant library of Salmonella Enteritidis (SE) was constructed through bacterial conjugation, followed by screening for motility-deficient mutants. Among 1321 mutants screened, C50041trmE::Tn5 exhibited reduced motility. To validate this phenotype, we constructed C50041ΔtrmE mutants and complementary strains C50041ΔtrmE::trmE. Compared to parental strain SE(C50041), C50041ΔtrmE displayed significantly lower mRNA levels of flagellar synthesis-related genes as determined via quantitative real-time PCR and the few visible flagella observed via transmission electron…
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Taxonomy
TopicsSalmonella and Campylobacter epidemiology · Bacteriophages and microbial interactions · Bacterial Genetics and Biotechnology