# Studies of Release Activity in DRGs of Rats with Spared Nerve Injury (SNI) Using YO-PRO-1

## TL;DR

This paper introduces a new method to study membrane permeability changes in rat DRG cells after nerve injury using a fluorescent dye.

## Contribution

The novel use of YO-PRO-1 to detect in vivo DRG cell release activity in injured neurons.

## Key findings

- YO-PRO-1 was taken up by injured neurons and surrounding non-neuronal cells in SNI rats.
- The method allows for in vivo examination of DRG cell release activity in intact animals.

## Abstract

We report a new method for examining changes in membrane permeability in dorsal root ganglion (DRG) cells under peripheral nerve injury conditions. By intravenously injecting YO-PRO-1, a monomeric cyanine nucleic acid stain, into rats with spared nerve injury (SNI), we observed the uptake of YO-PRO-1 in injured neurons and surrounding non-neuronal cells. This approach provides a tool for detecting in vivo DRG cell release activity in intact animals.