Disruption of CmHmgr1 triggers apoptosis and causes defects in growth, conidiogenesis, and mycoparasitism of Coniothyrium minitans
Xiaoxiang Yang, Haixuan Wang, Lei Zhang, Zhongmei Zhang, Zijin Hu, Daohong Jiang, Yanping Fu
TL;DR
Disrupting the CmHmgr1 gene in Coniothyrium minitans impairs its growth, spore production, and ability to parasitize other fungi.
Contribution
This study identifies CmHmgr1 as a key gene involved in conidiogenesis and mycoparasitism in C. minitans.
Findings
Disruption of CmHmgr1 leads to reduced hyphal development and conidiation in C. minitans.
CmHmgr1 is localized in mitochondria and its disruption triggers apoptosis.
The gene is essential for effective mycoparasitism of Sclerotinia sclerotiorum by C. minitans.
Abstract
Coniothyrium minitans is a well-known mycoparasite against Sclerotinia sclerotiorum. Two critical factors for the commercialization of C. minitans as a biocontrol agent are conidial production and parasitism. To decipher the mechanisms of conidiogenesis and mycoparasitism in C. minitans, a conidiation-deficient mutant, ZS-1TN5012, was isolated from a transfer DNA (T-DNA) insertional library. This mutant exhibited significantly reduced hyphal development, poor conidiation, and decreased sclerotial mycoparasitism. CmHmgr1 encoding a 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) was disrupted by the T-DNA insertion. The colony morphology of the wild-type strain ZS-1 resembled that of the mutant ZS-1TN5012 when the HMGR inhibitor atorvastatin was added to potato dextrose agar, with the mutant showing more sensitivity to atorvastatin. Furthermore, cellular localization assays…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsPlant Pathogens and Fungal Diseases · Plant pathogens and resistance mechanisms · Plant-Microbe Interactions and Immunity