Genome-Wide Analysis of KCS Gene Family in Ginkgo biloba L. and Functional Identification of KCS7 in Oleic Acid Synthesis
Xingyu Zhang, Kaifang Fan, Zhi Feng, Zhi Yao, Jinyuan Li, Shuguang Zhang, Xiaoqin Mi, Fuwen Wu, Yiqiang Wang, Meng Li
TL;DR
This study identifies a key gene in Ginkgo biloba involved in producing oleic acid, a vital fatty acid for plant growth and stress resistance.
Contribution
The study identifies GbKCS7 as a key gene in oleic acid synthesis in Ginkgo biloba through functional validation in yeast and Arabidopsis.
Findings
GbKCS7 overexpression in yeast increased C18:1 content by 2.07-fold.
GbKCS7 overexpression in Arabidopsis increased C18:1 by 27.70–31.43%.
GbKCS7 also increased very long-chain fatty acids (VLCFAs) in transgenic plants.
Abstract
Background: β-ketolipoyl coenzyme A synthase (KCS) is an essential limiting catalyst involved in carbon chain elongation during fatty acid biosynthesis, characterized by strict substrate specificity. C18:1 (oleic acid) plays a vital role in cell membranes and is essential for nutrient storage and stress defense. There are indications of significant accumulation and rapid synthesis of C18:1 during the early growth stages of Ginkgo biloba L. episperm. The KCS gene family in G. biloba has yet to be analyzed, and the role of KCS in oleic acid synthesis remains unexplored. Methods: In this study, this issue was investigated using transcriptomic and metabolomic data, bioinformatics analysis to screen a key gene from the KCS gene family, and dual validation using yeast and Arabidopsis thaliana expression systems to probe its function. Results: A total of 11 members of the GbKCS gene family…
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Taxonomy
TopicsLipid metabolism and biosynthesis · Plant biochemistry and biosynthesis · Biofuel production and bioconversion