Do NGF and LPS Interact Synergistically to Modulate Inflammation in Sheep Endometrial Epithelial Cells?

TL;DR

This study investigates how NGF and LPS affect inflammation in sheep endometrial cells, finding that NGF drives gene expression while LPS may influence prostaglandin production.

Contribution

The study reveals NGF as the primary transcriptional driver and suggests LPS may modulate prostaglandin output post-transcriptionally in endometrial cells.

Findings

NGF alone activates genes like NTRK1, COX2, and STAR in sheep endometrial cells.

LPS increases TLR4 and IGFBP6 but does not enhance transcription beyond NGF alone.

Combined NGF and LPS treatment modestly increases PGF2α production, suggesting post-transcriptional effects.

Abstract

Neurotrophins and inflammatory mediators are known to influence endometrial function, but their interplay in luminal epithelial cells remains poorly characterized. In this study, sheep endometrial luminal epithelial cells (SELECs) were treated with nerve growth factor (NGF), lipopolysaccharide (LPS), or both, and the effects on gene expression and prostaglandin secretion were evaluated. NGF stimulation alone induced a clear transcriptional activation of NGF, neurotrophic receptor tyrosine kinase 1 (NTRK1), p75 neurotrophin receptor (p75NTR), cyclooxygenase 2 (COX2), and steroidogenic acute regulatory protein (STAR). LPS treatment selectively increased Toll-like receptor 4 (TLR4), COX2, and insulin-like growth factor binding protein 6 (IGFBP6). Combined NGF and LPS treatment did not enhance the transcriptional response beyond that induced by NGF alone, except for STAR. However, co-treatment resulted in a modest increase in prostaglandin production, particularly prostaglandin F2α (PGF2α), but not prostaglandin E2 (PGE2), compared to single treatments, suggesting a possible post-transcriptional modulation rather than a transcriptional synergy. These findings indicate that NGF acts as the primary transcriptional driver in SELECs, while LPS contributes selectively and may enhance prostaglandin output. The observed increase in prostaglandin production may involve post-transcriptional mechanisms, although this remains to be confirmed.

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