Exploration of Hypolipidemic Effects of Sterols from Pleurotus tuber-regium(Fr.) Sing Sclerotium

TL;DR

This study explores how sterols from Pleurotus tuber-regium can reduce lipid levels in mice with high-fat diets.

Contribution

The study identifies optimal sterol extraction conditions and demonstrates their hypolipidemic effects in a mouse model.

Findings

High-dose sterol extract reduced body weight by 17.2% and liver weight by 30.9% in hyperlipidemic mice.

Serum low-density lipoprotein cholesterol decreased by 20.0% in the high-dose group.

Serum total and high-density lipoprotein cholesterol levels remained unchanged.

Abstract

The extraction technology of sterol was confirmed by ethanol reflux and saponification in this study. The orthogonal test was employed to assess the impact of extraction time, solid–liquid ratio, ethanol concentration and extraction temperature on the yield of sterol extraction. Hyperlipidemia model mice were established by feeding a high-fat and -sugar diet, and different doses of sterol extracts were given to the mice by gavages. The optimal extraction conditions were identified as an extraction time of 80 min, a solid–liquid ratio of 1:10, an ethanol concentration of 95%, and an extraction temperature of 90 °C, resulting in a sterol concentration of 1.16 mg/g. Compared with the high-fat model group, the high-dose group significantly reduced body weight by 17.2%, liver weight by 30.9%, and serum low density lipoprotein cholesterol by 20.0% (p < 0.05), while serum total cholesterol (5.59 ± 0.48 vs. 5.68 ± 0.64 mmol/L) and high-density lipoprotein cholesterol (0.98 ± 0.05 vs. 0.93 ± 0.03 mmol/L) showed no significant changes compared to the model group.

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