# CtGH76, a Glycoside Hydrolase 76 from Chaetomium thermophilum, with Elongated Glycan-Binding Canyon

## TL;DR

This paper studies a fungal enzyme, CtGH76, and finds it can bind to a variety of glycans due to its unique structure, offering new insights into fungal cell wall biology.

## Contribution

The study reveals the structural and functional divergence of CtGH76, a fungal GH76 enzyme, with a novel glycan-binding canyon.

## Key findings

- CtGH76 recognizes GPI-anchors similar to the Dfg5 subfamily.
- The enzyme shows promiscuity toward glycans with central α1,6-mannobiose motifs.
- An elongated glycan-binding canyon explains its broad substrate specificity.

## Abstract

Fungal cell walls, composed of polysaccharides and proteins, play critical roles in adaptation, cell division, and protection against environmental stress. Their polyglucan components are continuously remodeled by various types of glycosyl hydrolases (GHs) and transferases (GTs). In Saccharomyces cerevisiae and other ascomycetes, enzymes of the Dfg5 subfamily, which belong as GTs to the GH76 family, cleave an α1,4 linkage between glucosamine and mannose to facilitate covalent linkage of GPI-anchored proteins to the cell wall’s polyglucans. In contrast, the functions of other fungal GH76 subfamilies are not understood. We characterized CtGH76 from the sordariomycete Chaetomium thermophilum, a member of the Fungi/Bacteria-mixed GH76 subfamily, revealing conserved structural features and functional divergence within the GH76 family. Notably, our structural characterization by X-ray crystallography combined with glycan fragment screening indicated that CtGH76 can recognize GPI-anchors like members of the Dfg5 subfamily but shows a broader promiscuity toward other glycans with central α1,6-mannobiose motifs due to the presence of an elongated glycan-binding canyon. These findings provide new insights into GH76 enzyme diversity and fungal cell wall maturation.