# The Effect of Light on the Germination of Raphanus sativus Seeds and the Use of Sprout Extracts in the Development of a Dermatocosmetic Gel

## TL;DR

This study explores how different light sources affect radish sprout germination and uses the sprouts to create a natural skincare gel with antioxidant properties.

## Contribution

The novelty lies in using radish sprout extracts, influenced by light conditions, to develop a dermatocosmetic gel with antioxidant properties.

## Key findings

- Green LED light maximized sprout biomass, while red LED maximized polyphenol content.
- White LED light produced sprouts with the highest antioxidant activity.
- Radish sprout extracts were successfully formulated into a stable, pH-balanced dermatocosmetic gel.

## Abstract

This study investigates the influence of different light sources (sunlight, green, red, and white LED) on the germination of Raphanus sativus L. sprouts and the potential use of their sprout extracts in the development of natural dermatocosmetic gels. The bioactive fractions were extracted using simple methods and analyzed for total polyphenol content and antioxidant activity. Statistical analysis of weight, total phenolic content, and antioxidant activity of Raphanus sativus L. sprouts was performed using ANOVA. Sprouts exposed to green LED light showed the highest biomass (16.13 ± 0.38 g), while red LED light resulted in the highest total polyphenol content (3.28 ± 0.03 mg GAE/g fresh weight). The highest antioxidant activity (6.60 ± 0.08 mM Trolox/g fresh weight) was obtained under white LED. Although variations were observed, ANOVA analysis revealed that only sprout weight differed significantly among treatments (p < 0.001), while differences in polyphenol content and antioxidant activity were not statistically significant (p > 0.05). The extract with the highest antioxidant activity was incorporated as an active ingredient into Carbopol-based hydrogel formulations containing natural gelling agents and gentle preservatives. The resulting gels demonstrated favorable pH (4.85–5.05), texture, and stability. The results indicate that the light spectrum influences the germination process and the initial development of seedlings. Moreover, radish sprout extracts, rich in bioactive compounds, show promise for dermatocosmetic applications due to their antioxidant, soothing, and antimicrobial properties. This study supports the use of natural resources in the development of care products, in line with current trends in green cosmetics.