# Genome-Wide Analysis of the Typical Thioredoxin Gene Family in Hexaploid Oil-Camellia: Identification, Phylogenetic Analysis, and Gene Expression Patterns

## TL;DR

This study identifies and analyzes the thioredoxin gene family in oil-camellia, focusing on the role of one gene in controlling flowering.

## Contribution

The study provides a genome-wide analysis of the TRX gene family in hexaploid Camellia oleifera and identifies CoTRX25's role in flowering regulation.

## Key findings

- 27 typical TRX genes were identified and analyzed in the C. oleifera genome.
- Most TRX genes are highly expressed in embryos, suggesting a role in seed development.
- Overexpression of CoTRX25 in Arabidopsis delayed flowering, indicating its involvement in flowering regulation.

## Abstract

Hioredoxins are small proteins crucial for maintaining cellular redox balance and are involved in various biological processes, including growth, photosynthesis, development, and stress responses. This study aims to conduct a genome-wide analysis of the typical Thioredoxin (TRX) gene family in hexaploid Camellia oleifera and explore the role of the CoTRX25 gene in flowering. Through bioinformatics approaches, we identified 27 typical TRX gene family members in the C. oleifera genome and analyzed their phylogenetic relationships, gene structures, conserved motifs, and chromosomal distributions. Transcriptomic analysis across different tissues was performed to determine the expression patterns of these genes. Additionally, the CoTRX25 gene was cloned and heterologously overexpressed in Arabidopsis thaliana to investigate its functional role in flowering. The 27 TRX genes were mainly located on 11 chromosomes, with multiple gene duplication events identified, indicating that gene duplication has played a significant role in the expansion of the TRX family. Transcriptomic analysis revealed that most typical TRX genes are highly expressed in embryos, suggesting their potential importance in seed development. Overexpression of CoTRX25 in A. thaliana led to delayed flowering, implying that this gene may be involved in flowering regulation. This study provides a theoretical basis for understanding the functions of typical TRX genes in C. oleifera growth and development, particularly highlighting the role of CoTRX25 in flowering regulation.