# Hypergravity and ERK Inhibition Combined Synergistically Reduce Pathological Tau Phosphorylation in a Neurodegenerative Cell Model

## TL;DR

Hypergravity combined with ERK inhibition reduces harmful Tau protein changes in a lab model of neurodegeneration.

## Contribution

The synergistic effect of hypergravity and ERK inhibition on Tau phosphorylation is newly demonstrated in a neurodegenerative cell model.

## Key findings

- 50× g hypergravity combined with PD-0325901 reduced Tau aggregate area in HT22 cells.
- The treatment decreased phosphorylation at key Tau residues pS262 and pS396.
- Hypergravity may help reduce cytoskeletal damage caused by Tau aggregation.

## Abstract

This study evaluates the effects of hypergravity (HG) on a neurodegenerative model in vitro, looking at how HG influences Tau protein aggregation in Mouse Hippocampal Neuronal Cells (HT22) induced by neurofibrillary tangle seeds. Overall, 50× g significantly, synergistically, reduced the Tau aggregate Area when combined with ERK-inhibitor PD-0325901, correlating with decreased phosphorylation at critical residues pS262 and pS396. These findings suggest HG treatments may help mitigate cytoskeletal damage linked to Tau aggregation.