Immunosensor Enhanced with Silver Nanocrystals for On-Chip Prostate-Specific Antigen Detection
Timothy A. Okhai, Kefilwe V. Mokwebo, Marlon Oranzie, Usisipho Feleni, Lukas W. Snyman
TL;DR
This paper describes a new electrochemical immunosensor using silver nanocrystals to detect prostate-specific antigens with high sensitivity and selectivity.
Contribution
The novel contribution is the use of silver nanocrystals to enhance the performance of an on-chip immunosensor for PSA detection.
Findings
The immunosensor detected PSA with a detection limit of 1.14 ng/mL and an R2 of 0.99%.
The sensor showed good selectivity in the presence of interfering substances like glucose and urea.
The fabricated immunosensor demonstrated stability and repeatability comparable to existing technologies.
Abstract
An electrochemical immunosensor for the quantification of prostate-specific antigens (PSAs) using silver nanocrystals (AgNCs) is reported. The silver nanocrystals were synthesized using a conventional citrate reduction protocol. The silver nanocrystals were characterized using scanning electron microscopy (SEM) and field effect scanning electron microscopy (FESEM), X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), Fourier-transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, and small-angle X-ray scattering (SAXS). The proposed immunosensor was fabricated on a glassy carbon electrode (GCE), sequentially, by drop-coating AgNCs, the electro-deposition of EDC-NHS, the immobilization of anti-PSA antibody (Ab), and dropping of bovine serum albumin (BSA) to prevent non-specific binding sites. Each stage of the fabrication process was characterized by…
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Taxonomy
TopicsAdvanced biosensing and bioanalysis techniques · Gold and Silver Nanoparticles Synthesis and Applications · Advanced Nanomaterials in Catalysis