A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian Cells

TL;DR

This paper introduces a new microfluidic device that allows for long-term mammalian cell culture and dynamic media mixing, enabling precise in vitro studies of cell behavior.

Contribution

The first integration of a single-layer microfluidic mixing device with vacuum-assisted cell loading for mammalian cell culture.

Findings

The device efficiently mixes and dynamically exchanges media in cell-trapping chambers.

Mammalian cells remain viable during long-term culture in the device.

Finite element modeling accurately predicted flow rates and mixing performance.

Abstract

The possibility of tightly controlling the cellular microenvironment within microfluidic devices represents an important step toward precision analysis of cellular phenotypes in vitro. Microfluidic platforms that allow both long-term mammalian cell culture and dynamic modulation of the culture environment can support quantitative studies of cells’ responses to drugs. Here, we report the design and testing of a novel microfluidic device of simple production (single Polydimethylsiloxane layer), which integrates a micromixer with vacuum-assisted cell loading for long-term mammalian cell culture and dynamic mixing of four different culture media. Finite element modeling was used to predict flow rates and device dimensions to achieve diffusion-based fluid mixing. The device showed efficient mixing and dynamic exchange of media in the cell-trapping chambers, and viability of mammalian cells cultured for long-term in the device. This work represents the first attempt to integrate single-layer microfluidic mixing devices with vacuum-assisted cell-loading systems for mammalian cell culture and dynamic stimulation.

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