Physiological and Biochemical Responses and Transcriptome Analysis of Bangia fuscopurpurea (Rhodophyta) Under High-Temperature Stress

TL;DR

This study explores how a red alga, Bangia fuscopurpurea, responds to high temperatures by analyzing its growth, photosynthesis, and gene activity.

Contribution

The paper provides new insights into the molecular and physiological mechanisms of heat stress adaptation in Bangia fuscopurpurea.

Findings

Heat stress significantly reduced the relative growth rate of Bangia fuscopurpurea.

Transcriptomic analysis showed up-regulation of genes related to photosynthesis and antioxidant defenses under heat stress.

Photosynthetic parameters like Fv/Fm ratio decreased significantly under high temperature.

Abstract

With the advancement of human industrial activities, increased carbon dioxide emissions have made global warming an inescapable trend. Elevated temperatures exert profound effects on the viability of large macroalgae. Bangia fuscopurpurea (Rhodophyta) is a commercially important large red alga widely cultivated along the coastal waters of Putian, Fujian Province, China; however, its physiological, biochemical, and molecular responses to heat stress remain unclear. To address this question, we cultured B. fuscopurpurea at 15 °C (control) and 28 °C (heat stress) for 7 days, assessed changes in growth and photosynthetic parameters, and performed transcriptome sequencing. Growth analysis revealed that the relative growth rate of B. fuscopurpurea at 28 °C was significantly lower than that at 15 °C. After 1 day at 28 °C, the chlorophyll a and carotenoid contents increased significantly; the phycobiliprotein levels rose markedly on days 4 and 7, whereas the Fv/Fm ratio decreased significantly on days 1, 4, and 7. Transcriptomic analysis indicated that heat stress up-regulated the majority of differentially expressed genes (DEGs) in B. fuscopurpurea. KEGG pathway enrichment analysis revealed that the DEGs were predominantly associated with photosynthesis, carbohydrate and energy metabolism, glycerophospholipid metabolism, and the glutathione cycle. In summary, B. fuscopurpurea mitigates the adverse effects of heat stress by up-regulating genes involved in photosynthesis, antioxidant defenses, and glycerophospholipid metabolism. These findings enhance our understanding of the physiological adaptations and molecular mechanisms by which B. fuscopurpurea responds to heat stress.

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