# Tri-Layered Full-Thickness Artificial Skin Incorporating Adipose-Derived Stromal Vascular Fraction Cells, Keratinocytes, and a Basement Membrane

## TL;DR

This study develops a tri-layered artificial skin using cells and a basement membrane to improve wound healing without surgery.

## Contribution

A novel tri-layered artificial skin combining SVF cells, keratinocytes, and a collagen-elastin basement membrane is proposed.

## Key findings

- The basement membrane supported cell attachment without significant cytotoxic effects.
- The artificial skin showed structural and mechanical properties suitable for clinical wound healing.
- The method overcomes limitations of current artificial skin technologies.

## Abstract

Tissue-engineered artificial skin has the potential to enhance wound healing without necessitating extensive surgical procedures or causing donor-site morbidity. The purpose of this study was to examine the possibility of developing tri-layered tissue-engineered full-thickness artificial skin with a basement membrane for clinical use to accelerate wound healing. We engineered full-thickness artificial skin with a basement membrane for wound healing by employing stromal vascular fraction (SVF) cells for the dermal layer and autologous keratinocytes for the epidermal layer. The fabrication of a basement membrane involved the use of 100% bovine collagen and 4% elastin produced through a low-temperature three-dimensional printer. Scaffolds for cells were printed with 100% bovine collagen. The basement membrane underwent evaluations for collagenase degradation, tensile strength, and structural characteristics using scanning electron microscopy. The final tri-layered full-thickness artificial skin included two cell scaffolds with a basement membrane between them. The basement membrane may support cellular attachment without inducing significant cytotoxic effects. This study presents a novel strategy for full-thickness artificial skin development, combining SVF and keratinocytes with an optimized collagen-elastin basement membrane. This method may overcome the significant limitations of current artificial skin, thereby contributing to the advancement of tissue-engineering in wound healing for clinical use.