# Lipid Profile Characterization of Human Micro-Fragmented Adipose Tissue via Untargeted Lipidomics

## TL;DR

This study compares the lipid profiles of human lipoaspirate and micro-fragmented adipose tissue to understand changes during processing for regenerative medicine.

## Contribution

The study provides a novel untargeted lipidomic comparison of LA and MFAT, revealing lipid subclass differences with potential clinical implications.

## Key findings

- MFAT shows higher representation of diacylglycerols, triacylglycerols, phospholipids, and sphingolipids compared to LA.
- MFAT has lower abundance of monounsaturated fatty acids based on targeted fatty acid analysis.

## Abstract

Mesenchymal stem cells (MSCs) exhibit low immunogenicity, multipotency, and are abundantly present in adipose tissue, making this tissue an easily accessible resource for regenerative medicine. Different commercial procedures have been developed to micro-fragment the adipose tissue aspirate from patients before its reinjection. We explored a commercial device which mechanically micro-fragments human lipoaspirate (LA) resulting in a homogeneous micro-fragmentation of fat tissue (MFAT). This device has been successfully employed in several clinical applications involving autologous adipose tissue transplantation. Here, we compare the untargeted/targeted lipidomic profile of LA and MFAT looking for differences in terms of qualitative modifications occurring during the handling of the original LA material. In MFAT, different lipid subclasses such as diacylglycerols, triacylglycerols, phospholipids, and sphingolipids are more represented than in LA. In addition, via targeted fatty acids analysis, we found a lower abundance of monounsaturated fatty acids in MFAT. The biological implications of these findings must be better investigated to contribute to a better understanding of the clinical efficacy of MFAT and for its potential use as a scaffold for drug delivery applications.