# Analysis of serum metabolomics in patients with different types of chronic heart failure

## TL;DR

This study identifies potential blood metabolite biomarkers and metabolic pathways that can distinguish between different types of chronic heart failure.

## Contribution

The study presents seven potential metabolite biomarkers and highlights the glycerol phospholipid metabolism pathway as a key differentiator for heart failure subtypes.

## Key findings

- Blood metabolite profiles of HFpEF, HFmrEF, and HFrEF groups were clearly distinguishable.
- Seven potential biomarkers, including phosphatidylcholine and ceramide, were identified.
- The glycerol phospholipid metabolic pathway was found to be the most significant in differentiating heart failure subtypes.

## Abstract

Heart failure remains a major public health issue, and there are still no reliable biomarkers for left ventricular ejection fraction (LVEF).

To screen for differential metabolites in the blood of HFpEF, HFmrEF, and HFrEF patients based on metabolomics analysis of their blood samples.

Total 44 patients in HFpEF group, 30 patients in HFmrEF group, and 36 patients in HFrEF group were selected. The blood metabolites were analyzed by liquid chromatography high-resolution mass spectrometry and classified by principal component analysis, and then potential biomarker were screened. Partial least squares discriminant analysis was used to model and investigate the predictive ability of biomarkers for LVEF.

Blood metabolite profiles of HFpEF, HFmrEF, and HFrEF groups could be well distinguished, and seven potential biomarkers were identified, such as phosphatidylcholine, phosphatidylinositol, lysophosphatidylcholine, lysophosphatidylcholine, ceramide, sphingosine, and sphingomyelin. Four metabolic pathways, such as glycerol phospholipid metabolic pathway, linoleic acid metabolic pathway, purine pyrimidine metabolism pathway, and linolenic acid metabolism pathway were identified, among which glycerol phospholipid metabolism pathway was the most significant.

The changes in glycerol phospholipid metabolism pathway may help identify HFpEF, HFmrEF, and HFrEF.