# Comparison of Hemostatic Activity in Canine Leukoreduced Cryoprecipitate, Cryopoor Plasma, and Fresh Plasma

## TL;DR

This study compares the clotting abilities of different types of canine blood products and finds that cryoprecipitate has higher Factor VIII, which is important for blood clotting.

## Contribution

The study is the first to evaluate hemostatic activity in canine leukoreduced cryoprecipitate and cryopoor plasma, providing new insights into their clinical utility.

## Key findings

- Factor VIII activity is significantly higher in LR-CRYO compared to LR-FP.
- Refrozen LR-CRYO shows no significant differences in most hemostatic factors compared to LR-CRYO.
- LR-CPP has higher Factor X activity and albumin concentration but lower von Willebrand Factor and fibrinogen.

## Abstract

To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR‐CRYO) and leukoreduced cryopoor plasma (LR‐CPP).

We aimed to compare the hemostatic activity of LR‐CRYO and LR‐CPP to leukoreduced fresh plasma (LR‐FP) and to evaluate the preservation of LR‐CRYO by refrigeration and refreezing after thawing.

Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR‐FP, then frozen (−20°C) to obtain leukoreduced fresh frozen plasma (LR‐FFP). LR‐FFP was further separated into LR‐CRYO and LR‐CPP. LR‐CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24 h and one refrozen for 7 days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D‐dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR‐FP, LR‐CRYO, LR‐CPP, refrigerated, and refrozen LR‐CRYO.

FVIII activity was higher in LR‐CRYO (p = 0.0001) versus LR‐FP. vWF activity (p < 0.0001) and fibrinogen concentration (p = 0.0012) were lower in LR‐CPP versus LR‐FP. FX activity was higher in LR‐CPP (p < 0.0001) and LR‐FP (p = 0.0002) versus LR‐CRYO, and albumin concentration was higher in LR‐CPP versus LR‐FP (p < 0.0001) and LR‐CRYO (p < 0.0001). No statistically significant difference was found in refrigerated or refrozen LR‐CRYO as compared with LR‐CRYO, excluding ATA, which was lower (p = 0.0062) in refrigerated LR‐CRYO.

Because the concentration of FVIII is higher in LR‐CRYO than in LR‐FP, LR‐CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR‐CRYO as compared with LR‐CRYO, LR‐CRYO can be frozen after thawing for reuse.