Micro-scale genetic structure and genetic variation of Neotricula aperta (Gastropoda: Pomatiopsidae), the intermediate host of Schistosoma mekongi (Digenea: Schistosomatidae) in Champasak Province, Laos

TL;DR

This study examines the genetic diversity and population structure of a freshwater snail in Laos that hosts a parasite causing a type of schistosomiasis.

Contribution

The study provides new insights into the micro-scale genetic structure of Neotricula aperta in Champasak Province, Laos.

Findings

High haplotype diversity (0.996 for cox1 and 0.911 for 16S rRNA) was observed in N. aperta populations.

Significant genetic differentiation was found among populations at different spatial scales.

Limited gene flow and potential geographic barriers are likely contributing to the observed genetic structure.

Abstract

Neotricula aperta, a freshwater snail found in the Mekong River, serves as the intermediate host of the blood fluke Schistosoma mekongi, the causative agent of schistosomiasis mekongi in Cambodia and Laos. Understanding the genetic diversity, population structure of this snail in relation to its geographical distribution is crucial for a comprehensive understanding of disease transmission. In this study, we investigated the genetic diversity, and genetic structure of N. aperta in Champasak Province, Laos.

A total of 80 N. aperta snails were collected from 13 various localities across five villages in Khong and Mounlapamok districts in Champasak Province, Laos in May 2024. Species of snails were initially identified based on morphology and subsequently confirmed by DNA barcoding. Molecular analyses were conducted using specific primers to amplify two mitochondrial DNA genes, namely cytochrome c oxidase subunit 1 (cox1) and 16S ribosomal RNA (16S rRNA), in order to assess the genetic diversity of N. aperta populations.

Based on cox1 sequences, the overall haplotype diversity was 0.996, while the overall nucleotide diversity was 0.049. For the 16S rRNA marker, the overall haplotype diversity was 0.911, and the overall nucleotide diversity was 0.015. Analysis of molecular variance (AMOVA) confirmed significant genetic differentiation (P-value < 0.05) among populations at different spatial scales, including villages, catchments, and countries. This genetic structure likely reflects limited gene flow among snail populations, potentially due to geographical barriers. Although local environmental factors may also contribute to differentiation, the current genetic data are insufficient to distinguish between geographic isolation and adaptive divergence. Further ecological and functional investigations will be needed to determine whether adaptive processes are influencing population structure.

The genetic divergence of N. aperta observed in this study indicates a high level of genetic differentiation both among and within populations. This pattern suggests that N. aperta is possibly undergoing localized adaptation or that barriers to gene flow, such as physical, ecological, or behavioral factors, are promoting the accumulation of genetic differences. Micro-scale genetic structuring within populations may be driven by microhabitats or small-scale ecological gradients, while limited dispersal, localized mating preferences, or other behavioral traits may contribute to differentiation among populations.

The online version contains supplementary material available at 10.1186/s41182-025-00775-9.

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