A spurious fln-2 mutation in a wide variety of commonly used C. elegans strains

TL;DR

A mutation in the fln-2 gene is found in many C. elegans strains, including those used for transgenic research, affecting a wide range of studies.

Contribution

The study identifies a fln-2 mutation in a widely used C. elegans strain and its descendants, expanding its known impact.

Findings

The fln-2 mutation is present in the dpy-5(e907) strain, a common ancestor for many transgenic lines.

This mutation is now found in thousands of transgenic C. elegans strains used in research.

The mutation was previously identified in a wild-type male stock strain used for outcrossing.

Abstract

We describe a

fln-2

mutant allele present in many commonly used

Caenorhabditis elegans

strains. It is present in the

dpy-5
(
e907
)

strain, ancestral to thousands of transgenic strains generated by the

C. elegans

Expression Project. This finding broadens the number of strains affected by the

fln-2

mutation, now impacting thousands of transgenic lines used in diverse studies. This expands on the previous identification of

the
fln-2

mutation in a wild-type male stock strain used for outcrossing in genetic studies.

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