Chromosome-level genome assembly of the large carpenter bee Xylocopa dejeanii Lepeletier, 1841 (Hymenoptera: Apidae)

TL;DR

This paper presents the first chromosome-level genome assembly of the large carpenter bee Xylocopa dejeanii, enhancing understanding of bee biology and evolution.

Contribution

The study provides the first pseudo-chromosome-level genome assembly for Xylocopa dejeanii using advanced sequencing technologies.

Findings

The final genome is 194.44 Mb, assembled into 16 chromosomes with 99.00% completeness.

The assembly identified 10,970 protein-coding genes and 432 ncRNAs.

The study advances Xylocopinae genomics and offers insights into bee evolution.

Abstract

Xylocopinae, a diverse bee subfamily comprising over 1,000 bee species, and also a major model system for studying the pollination and evolution of sociality. The lack of chromosome-level genome assembly resources for the Xylocopinae limits our research of their biology and evolution. Here, we provided the first pseudo-chromosomes genome assembly of the Xylocopa dejeanii combined PacBio CLR long reads, Illumina sequences, and Hi-C data. The final genome is 194.44 Mb located in 16 chromosomes. Our assembly includes 141 scaffolds, with a scaffold N50 length of 13.15 Mb. BUSCO analysis revealed 99.00% completeness. Genome annotation identified 28.27 Mb of repetitive elements, 10,970 protein-coding genes, and 432 ncRNAs. This high-quality X. dejeanii assembly advances our understanding of Xylocopinae genomics and provides new insights into bee evolution.

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