# Exploring Epigenetic Ageing Using Direct Methylome Sequencing

## TL;DR

This paper introduces a new method using nanopore sequencing to study DNA methylation patterns related to aging, offering a more detailed and efficient approach than previous techniques.

## Contribution

The novel framework utilizes nanopore sequencing to capture full CpG context and study epigenetic aging without bisulfite conversion.

## Key findings

- Nanopore sequencing captures full CpG context and diverse genomic regions for age-associated methylation profiling.
- The method removes bisulfite conversion steps and is compatible with the latest reference genome.
- Nanopore sequencing is established as a powerful tool for epigenetic aging research and anti-aging interventions.

## Abstract

Background/Objectives: Advances in nanopore sequencing have opened new avenues for studying DNA methylation at single-base resolution, yet their application in epigenetic ageing research remains underdeveloped. Methods: We present a novel framework that leverages the unique capabilities of nanopore sequencing to profile and interpret age-associated methylation patterns in native DNA. Results: Unlike conventional array-based approaches, long reads sequencing captures full CpG context, accommodates diverse and repetitive genomic regions, removes bisulfite conversion steps, and is compatible to the latest reference genome. Conclusions: This work establishes nanopore sequencing as a powerful tool for next-generation epigenetic ageing studies, offering a scalable and biologically rich platform for anti-ageing interventions monitoring and longitudinal ageing studies.