Proteoform Patterns in Hepatocellular Carcinoma Tissues: Aspects of Oncomarkers
Elena Zorina, Natalia Ronzhina, Olga Legina, Nikolai Klopov, Victor Zgoda, Stanislav Naryzhny
TL;DR
This study identifies distinct proteoform patterns in liver cancer and non-cancerous tissues, highlighting potential oncomarkers for hepatocellular carcinoma.
Contribution
The study introduces a comprehensive top-down proteomics approach to identify proteoform patterns as potential biomarkers in liver cancer.
Findings
Over 2500 proteoform patterns were visualized per sample type, revealing distinct protein signatures in HCC and nonmalignant tissues.
38 proteins showed significant differences in proteoform patterns between cancerous and non-cancerous liver tissues.
Most proteoform patterns were similar to theoretical unmodified proteins, but some showed altered patterns in cancer.
Abstract
Background: Human proteins exist in numerous modifications—proteoforms—which are promising targets for biomarker studies. In this study, we aimed to generate comparative proteomics data, including proteoform patterns, from hepatocellular carcinoma (HCC) and nonmalignant liver tissues. Methods: To investigate protein profiles and proteoform patterns, we employed a panoramic, integrative top-down proteomics approach: two-dimensional gel electrophoresis (2DE) coupled with liquid chromatography–electrospray ionization–tandem mass spectrometry (LC-ESI-MS/MS). Results: We visualized over 2500 proteoform patterns per sample type, enabling the identification of distinct protein signatures and common patterns differentiating nonmalignant and malignant liver cells. Among these, 1270 protein patterns were uniformly observed across all samples. Additionally, 38 proteins—including pyruvate kinase…
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Taxonomy
TopicsAdvanced Proteomics Techniques and Applications · Cancer, Hypoxia, and Metabolism · Glycosylation and Glycoproteins Research