Contrasting patterns in diversity and community assembly of bacterioplankton and three size fractions of protists in the South China Sea

TL;DR

The study explores how different sizes of marine microbes in the South China Sea form communities and how their size and feeding habits influence these patterns.

Contribution

The study reveals how cell size and trophic mode influence microbial community assembly in the South China Sea, challenging existing hypotheses.

Findings

Protist communities show distinct trophic modes across size fractions, with parasitic pico-protists and mixotrophic nano- and micro-protists.

Bacterial and pico-protist communities exhibit stronger vertical stratification due to niche breadth and dispersal patterns.

Stochastic processes dominate microbial community assembly, with homogeneous selection more prominent in nano-protist communities.

Abstract

The microbial food web plays a critical role in marine ecosystems, composed of various cell sizes of microbial organisms. Here, high-throughput sequencing of the 16S and 18S rRNA genes was conducted to detect the community structure and distribution patterns of bacterioplankton (0.2 µm–2 µm) and three size fractions of protist communities, i.e., pico-protist (0.2 µm–2 µm), nano-protist (2 µm–20 µm), and micro-protist (20 µm–200 µm), in the euphotic zone of the South China Sea. The trophic mode compositions of protist communities varied significantly across three size fractions, characterized by a substantial prevalence of parasitic pico-protists (40% amplicon sequence variants) and a greater predominance of mixotrophic taxa within nano- and micro-protist communities. Furthermore, we detected stronger vertical stratification of bacterial and pico-protist communities, corresponding to the wider niche breadth of smaller cells and reliance on passive dispersal. Additionally, both bacterial and protist community assemblies were dominated by stochastic processes. The relative contribution of homogeneous selection in nano-protist community assembly was greater compared to other size fractions, probably related to high relative abundance of mixotrophs. In summary, our results suggest that both cell size and trophic mode affect marine microbial community assembly, and that neither the “size-plasticity” hypothesis nor the “size-dispersal” hypothesis fully matched microbial communities. Our analyses are important for a better understanding of the assemblage processes of marine epipelagic microbial communities and how they will respond to global change.

Cell size is a key feature that influences microbial biology at both the cellular and community levels. Poorly understood is the extent to which diverse ecological factors influence the assembly of microbial communities of various sizes. Two important hypotheses addressing the mechanisms of biome assembly are “size-plasticity” and “size-dispersal.” Here, we investigated epipelagic microbial communities to reveal differences in the ecological functions of various microbial sizes, to explore the association of ecological processes with niche and cell size, and to expand the current understanding of marine microbial community assemblages and their possible responses to future global change.

Figures