# A chaperonin complex regulates organelle proteostasis in malaria parasites

## TL;DR

This study reveals how a chaperonin complex helps maintain protein stability in the apicoplast of malaria parasites and suggests new drug targets for treating malaria.

## Contribution

The discovery of a conserved chaperonin complex regulating apicoplast proteostasis and its role in stabilizing the Clp proteolytic complex.

## Key findings

- Ablation of CPN60 causes immediate parasite death due to apicoplast damage.
- CPN60 interacts with and stabilizes the Clp proteolytic complex in the apicoplast.
- Inhibitors of bacterial CPN60 show anti-Plasmodium activity beyond apicoplast function.

## Abstract

The apicoplast of Plasmodium parasites serves as a metabolic hub that synthesize essential biomolecules. Like other endosymbiotic organelles, 90% of the apicoplast proteome is encoded by the cell nucleus and transported to the organelle. Evidence suggests that the apicoplast has minimal control over the synthesis of its proteome and therefore it is unclear how organelle proteostasis is regulated. Here, we identified and investigated a large and conserved chaperonin (CPN) complex with a previously unknown function. Using genetic tools, we demonstrated that ablation of the apicoplast CPN60 subunit leads to parasite death due to organellar damage, immediately within its first replication cycle, deviating from the delayed death phenotype commonly observed for apicoplast translation inhibitors. Unlike its close orthologues in other prokaryotic and eukaryotic cells, CPN60 is not upregulated during heat shock (HS) and does not affect HS response in the parasite. Instead, we found that it is directly involved in proteostasis through interaction with the Clp (caseinolytic protease) proteolytic complex. We showed that CPN60 physically binds both the active and inactive forms of the Clp complex, and manipulates its stability. A computational structural model of a possible interaction between these two large complexes suggests a stable interface. Finally, we screened a panel of inhibitors for the bacterial CPN60 orthologue GroEL, to test the potential of chaperonin inhibition as antimalarial. These inhibitors demonstrated an anti-Plasmodium activity that was not restricted to apicoplast function, with additional targets outside of this organelle. Taken together, this work reveals how balanced activities of proteolysis and refolding safeguard the apicoplast proteome, and are essential for organelle biogenesis.

The cell of the human malaria parasite Plasmodium falciparum has a unique organelle called the apicoplast that produces essential metabolites, but it is unclear how it maintains a stable proteome. Here, we address the question of organelle proteostasis by investigating the function of a large chaperonin complex and its main subunit CPN60. We show that CPN60 mutants die due to organellar damage immediately within the first replication cycle, avoiding the typical apicoplast-delayed cell death. We demonstrate that it binds and stabilizes another large proteolytic complex and use computational tools to predict how a stable interface is attained. We use bacterial inhibitors to explore their potential as an antimalarial drug target. This study reveals how balanced refolding and proteolysis safeguard the apicoplast proteome and opens a new avenue for antimalarial drug discovery.