# Stability of Spiked Chlamydia Trachomatis and Neisseria Gonorrhea in Urine and Swab Specimens After Prolonged Storage at Room and Freezer Temperatures Using Aptima Combo-2 Test

## TL;DR

This study shows that Chlamydia and Neisseria can be reliably detected in stored urine and swab samples for up to 36 months using the Aptima Combo-2 test.

## Contribution

Demonstrates long-term stability of Chlamydia trachomatis and Neisseria gonorrhoeae in stored specimens using the Aptima Combo-2 assay.

## Key findings

- Specimens stored at room temperature remained CT/GC positive for 36 months.
- Specimens stored at −80°C also remained CT/GC positive for 36 months.
- Aptima Combo-2 assay reliably detected CT/GC in both storage conditions.

## Abstract

We evaluated whether prolonged storage at room and freezer temperatures affects detection of Chlamydia trachomatis and Neisseria gonorrhoeae (CT/GC) using Aptima Combo-2 assay for research studies. Three hundred specimens were spiked with CT/GC; half were stored at room temperature and half at −80°C. All specimens remained CT/GC positive for 36 months.