# Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT and UV-vis spectroscopy

## TL;DR

This paper provides a detailed protocol for measuring iodine uptake in sheep and pig meniscus tissue using micro-CT and UV-vis spectroscopy.

## Contribution

The study introduces a combined protocol using contrast-enhanced micro-CT and UV-vis spectroscopy to evaluate iodine dynamics in meniscus tissue.

## Key findings

- A staining procedure with iodine-based contrast agents was described for meniscus tissue.
- Sequential micro-CT scans over 24 days track iodine uptake dynamics.
- Radiodensity analysis and UV-vis spectroscopy quantify iodine uptake effectively.

## Abstract

Here, we present a protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-computed tomography (CT) and ultraviolet-visible (UV-vis) spectroscopy. We describe steps for sample fixation, a staining procedure with iodine-based contrast agents, and imaging using micro-CT. We then detail procedures for volumetric and radiodensity analyses, UV-vis spectroscopy, and data processing.

For complete details on the use and execution of this protocol, please refer to Orellana et al.1

•Assess iodine uptake and radiodensity via iodine-based staining and micro-CT imaging•Guidance for sequential micro-CT scans over 24 days to track iodine uptake dynamics•Integration of radiodensity analysis and UV-vis spectroscopy to quantify iodine uptake•Steps for Avizo and MATLAB for segmentation, volumetric analysis, and data visualization

Assess iodine uptake and radiodensity via iodine-based staining and micro-CT imaging

Guidance for sequential micro-CT scans over 24 days to track iodine uptake dynamics

Integration of radiodensity analysis and UV-vis spectroscopy to quantify iodine uptake

Steps for Avizo and MATLAB for segmentation, volumetric analysis, and data visualization

Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Here, we present a protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-computed tomography (CT) and ultraviolet-visible (UV-vis) spectroscopy. We describe steps for sample fixation, a staining procedure with iodine-based contrast agents, and imaging using micro-CT. We then detail procedures for volumetric and radiodensity analyses, UV-vis spectroscopy, and data processing.