# Serum cytokine profiles in patients with myasthenia gravis

## TL;DR

This study finds that patients with unstable myasthenia gravis have higher levels of certain cytokines in their blood compared to healthy people, suggesting these proteins could help classify patients.

## Contribution

The study identifies specific serum cytokine profiles in unstable myasthenia gravis patients, potentially offering new biomarkers for clinical stratification.

## Key findings

- Unstable MG patients had significantly higher serum levels of IL-1β, IL-2, IL-10, and IL-17 compared to healthy controls.
- AChR antibody-negative MG patients showed higher IL-1β and IL-5 levels than AChR antibody-positive patients.
- Cytokine levels in AChR antibody-positive MG patients decreased significantly with improvement in condition.

## Abstract

Cytokines play a crucial role in instigating inflammation and generating pathogenic autoantibodies at the neuromuscular junction in individuals suffering from myasthenia gravis (MG). The objective of this study is to investigate the cytokine profiles among patients grappling with MG.

This study recruited patients with unstable MG and healthy controls from the First Affiliated Hospital of Fujian Medical University during the period spanning January 2021 to December 2022. We evaluated IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12P70, IL-17, IFN-γ, IFN-α, and TNF-α in the serum using the Flow Cytometric Bead Array (CBA) technique.

A total of 104 patients and 54 healthy controls were included in the study. Notably, serum levels of interleukin (IL)-1β, IL-2, IL-10, and IL-17 exhibited significant elevation in unstable MG patients when compared to the healthy control group. Furthermore, levels of IL-1β and IL-5 were notably higher in unstable MG patients who tested negative for acetylcholine receptor (AChR) antibodies when compared to their AChR-antibody positive counterparts (P < 0.05). In AChR-antibody positive patients, there was a statistically significant decrease in IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL12-P70, IFN-γ, and IFN-α upon improvement. There was no discernible variation in MG patients at an unstable stage regardless of their onset time. Additionally, there was no statistically significant differences between pre- and post-thymectomy in thymoma-associated MG (TAMG).

Individuals with unstable MG appear to demonstrate elevated levels of serum IL-1β, IL-2, IL-10, and IL-17 compared to healthy individuals. Furthermore, among MG subgroups, those testing negative for antibodies, tend to display increased levels of IL-1β and IL-5. These serum cytokine profiles may hold promise as potential biomarkers for stratifying MG patients in clinical settings.