# The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post‐Thaw Sperm Quality of Kajli Ram Semen

## TL;DR

Adding 0.5% cinnamaldehyde to a semen extender improves the quality of frozen Kajli ram sperm by protecting the sperm membrane and viability.

## Contribution

This study demonstrates that 0.5% cinnamaldehyde in a semen extender significantly enhances post-thaw sperm quality in Kajli rams.

## Key findings

- 0.5% cinnamaldehyde significantly improved post-thaw sperm plasma membrane integrity.
- Sperm viability was significantly higher in the 0.5% cinnamaldehyde group compared to other concentrations.
- Cinnamaldehyde at 0.5% effectively preserved the quality of frozen Kajli ram sperm.

## Abstract

Cinnamaldehyde, a component of cinnamon bark extract, acts as a potential antioxidant by enhancing the activities of catalase, superoxide dismutase and glutathione peroxidase in various cell and animal models. This study aimed to evaluate the protective effects of cinnamaldehyde on the quality of Kajli ram sperm at 4°C and after freezing. Semen samples were obtained from 12 Kajli rams through an artificial vagina maintained at 42°C. Each ejaculate was divided into five aliquots and extended in a tris‐citric acid extender with different concentrations of cinnamaldehyde (0%, 0.5%, 1.0%, 2.5% and 5.0%). Post‐thaw semen quality was assessed. These results showed that the extender containing 0.5% cinnamaldehyde exhibited significant protective effects (p < 0.05) on post‐thaw sperm plasma membrane and sperm viability compared to the control and other experimental groups. In conclusion, supplementing the extender with 0.5% cinnamaldehyde effectively preserved the quality of post‐thawed sperm from Kajli rams.

This study evaluated the protective effects of cinnamaldehyde on Kajli ram sperm quality at 4°C and after freezing, using semen samples from 12 rams collected via an artificial vagina at 42°C. The semen was extended in a tris‐citric acid extender with varying cinnamaldehyde concentrations (0%, 0.5%, 1.0%, 2.5% and 5.0%), and post‐thaw quality was assessed. Results indicated that 0.5% cinnamaldehyde significantly improved sperm plasma membrane integrity and viability (p < 0.05), effectively preserving post‐thaw sperm quality.