# A novel method for the detection of Cas9 gRNAs using a fluorophore-labeled DNA oligo

**Authors:** Ranmal Avinash Bandara, Zhichang Peter Zhou, Ziyan Rachel Chen, Rongqi Duan, Alan Richard Davidson, Amy P. Wong, Jim Hu

PMC · DOI: 10.1016/j.gendis.2025.101636 · Genes & Diseases · 2025-04-14

## Full-text entities

- **Genes:** ADA (adenosine deaminase) [NCBI Gene 100] {aka ADA1}, BCAR1 (BCAR1 scaffold protein, Cas family member) [NCBI Gene 9564] {aka CAS, CAS1, CASS1, CRKAS, P130Cas}
- **Diseases:** genetic diseases (MESH:D030342), Cancer (MESH:D009369)
- **Chemicals:** adenine (MESH:D000225), cytosine (MESH:D003596), nucleotides (MESH:D009711), Cy5.5 (MESH:C098793), polyacrylamide (MESH:C016679), hydrogen (MESH:D006859), Urea (MESH:D014508)
- **Species:** Hungerfordia sp. U (species) [taxon 563713]
- **Mutations:** F508del
- **Cell lines:** HEK293T — Homo sapiens (Human), Transformed cell line (CVCL_0063)

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## References

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Source: https://tomesphere.com/paper/PMC12270778