# Sildenafil effect on testosterone-induced prostate hypertrophy and relaxation of urinary bladder neck muscles

## TL;DR

This study shows that sildenafil reduces prostate size and relaxes bladder muscles in rabbits with testosterone-induced prostate enlargement.

## Contribution

The study demonstrates sildenafil's dual effect on prostate hypertrophy and bladder neck muscle relaxation in a testosterone-induced BPH model.

## Key findings

- Sildenafil reduced prostate weight by 65.34% in rabbits with BPH.
- Sildenafil induced 32.8% relaxation of phenylephrine-contracted bladder neck muscles.
- Histopathological changes from BPH were reversed by sildenafil treatment.

## Abstract

Previous studies have demonstrated the expression of phosphodiesterase-5 receptors in prostate tissue. In this study, we investigated the efficacy of sildenafil citrate in testosterone-induced benign prostate hyperplasia (BPH) in rabbits.

Prostate hyperplasia was induced using testosterone propionate for 8 weeks. Rabbits were divided into two groups: control and experimental. The experimental group was further subdivided into two subgroups: one subgroup was sacrificed after testosterone induction, while the other subgroup received sildenafil (5 mg/kg/day) via intragastric intubation for 8 weeks. The weight of the prostate and relaxation of the bladder neck muscle were assessed. Organ bath experiments evaluated the effect of sildenafil on phenylephrine-precontracted bladder neck muscle strips.

The mean prostate weight was reduced by 65.34 % after 8 weeks of treatment with sildenafil in animals with BPH. Sildenafil induced significant smooth muscle relaxation of the phenylephrine-contracted bladder neck muscle strips. The mean relaxation value was 2.11 ± 0.13, representing a 32.8 % reduction in contraction percentage. Maximal relaxation was produced at 5.0 × 10⁻⁶ M of sildenafil. Sildenafil induced significant relaxation of the phenylephrine-contracted bladder neck muscle strips. Adding the nitric oxide synthase inhibitor L-NAME inhibited relaxation, whereas sodium nitroprusside, a nitric oxide donor, increased it. Histopathological analysis showed increased papillary projections, acinar areas, and epithelial thickness in the testosterone-treated group. Sildenafil treatment reversed the hypertrophic and hyperplastic changes.

The results indicate that sildenafil may provide a dual function in the treatment of erectile dysfunction and the relief of urinary tract complications associated with prostatic hypertrophy.

•The efficacy of sildenafil citrate in testosterone-induced BPH in rabbits was investigated.•Sildenafil reduced prostate weight and reversed histological changes associated with hyperplasia.•Sildenafil induced relaxation of bladder neck smooth muscle strips in testosterone-treated rabbits.

The efficacy of sildenafil citrate in testosterone-induced BPH in rabbits was investigated.

Sildenafil reduced prostate weight and reversed histological changes associated with hyperplasia.

Sildenafil induced relaxation of bladder neck smooth muscle strips in testosterone-treated rabbits.